Abstract：The paper focused on the preparation of bamboo leaf enzyme and its use for improvement of alkaline soil planted with bamboo. The results showed that the pH value of the bamboo leaf enzyme, which contains rich nutrients, was relative low, while the pH value of the solid phase was 3.77-4.95, and the pH value of the liquid phase was 3.45-4.03. After application of the enzyme into the alkaline soil, the pH value of soil decreased significantly, the soil nutrient content increased, and the soil improvement effect was significant. Bamboo leaf enzyme also prolongs the green period of bamboo leaves and promotes the growth of bamboo plants. Bamboo leaf enzyme is a kind of environmentally friendly and low-cost soil improvement agent.