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世界竹藤通讯  2019, Vol. 17 Issue (2): 31-35     https://doi.org/10.13640/j.cnki.wbr.2019.02.007
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云南甜龙竹组培及组培苗移栽技术研究
钟永莉, 陈玟旭, 谭宏超
云南师范大学生命科学学院 昆明 650500
A Study of Tissue Culture and Seedling Transplanting Technology for Dendrocalamus brandiss
Zhong Yongli, Chen Wenxu, Tan Hongchao
College of Life Sciences, Yunnan Normal University, Kunming 650500, China
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摘要 选择6种类型的云南甜龙竹外植体,经不同浓度的升汞溶液消毒一定时间,在添加不同浓度6-BA的MS培养基中进行组培试验,以筛选云南甜龙竹组培繁殖的适宜条件;同时,以1年生云南甜龙竹组培苗为材料,在6种育苗基质和4种育苗环境下试验组培苗的生长情况,以筛选适宜组培苗生长的最佳条件。结果表明:云南甜龙竹组培繁育以1年生种子苗茎段作为外植体,用0.10%~0.15%的升汞消毒6~9 min,使用标准MS培养基,添加浓度为1.5~2.0 g/mL的6-BA,能够获得较高的芽诱导率;1年生组培苗移栽时,采用大棚套小棚的移栽环境,以植物碎沫作为栽培基质,苗木成活率较高,幼苗生长较好。
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钟永莉
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关键词 云南甜龙竹组织培养外植体芽诱导率组培苗移栽环境    
Abstract:Six types of explants were selected from Dendrocalamus brandiss and after disinfected with different concentrations of mercuric chloride solution for a certain period of time, they were cultivated in MS medium supplemented with different concentrations of 6-BA, in order to screen the appropriate conditions for tissue culture of D. brandiss. Meanwhile, 1-year-old tissue culture seedlings were tested under 6 kinds of seedling substrates and 4 different environmental conditions to screen the optimal conditions suitable for the growth of tissue culture seedlings. The results showed that the explants of young culm from 1-year-old seedlings which were disinfected with 0.10%-0.15% mercuric chloride for 6-9 min and cultivated in standard MS medium with 1.5-2.0 g/mL of 6-BA, could achieve higher bud induction rate. When the 1-year-old tissue culture seedlings were transplanted, the seedlings were covered in greenhouse and cultivated with the fine particles made from plant debris, which could achieved higher survival rate and better growth.
Key wordsDendrocalamus brandiss    tissue culture    explant    bud induction rate    tissue culture seedling    transplanting condition
     出版日期: 2019-06-04
基金资助:云南珍竹农业科技有限公司与马来西亚竹子绿世界有限公司资助项目。
通讯作者: 谭宏超(1963-),男,教授,从事竹产业教学研究开发工作。E-mail:ynbamboo@126.com     E-mail: ynbamboo@126.com
作者简介: 钟永莉(1995-),女,在校本科生,从事竹子组织培养研究。E-mail:2727141387@qq.com
引用本文:   
钟永莉, 陈玟旭, 谭宏超. 云南甜龙竹组培及组培苗移栽技术研究[J]. 世界竹藤通讯, 2019, 17(2): 31-35.
Zhong Yongli, Chen Wenxu, Tan Hongchao. A Study of Tissue Culture and Seedling Transplanting Technology for Dendrocalamus brandiss. World Bamboo and Rattan, 2019, 17(2): 31-35.
链接本文:  
http://www.cafwbr.net/CN/10.13640/j.cnki.wbr.2019.02.007      或      http://www.cafwbr.net/CN/Y2019/V17/I2/31
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