Abstract：Six types of explants were selected from Dendrocalamus brandiss and after disinfected with different concentrations of mercuric chloride solution for a certain period of time, they were cultivated in MS medium supplemented with different concentrations of 6-BA, in order to screen the appropriate conditions for tissue culture of D. brandiss. Meanwhile, 1-year-old tissue culture seedlings were tested under 6 kinds of seedling substrates and 4 different environmental conditions to screen the optimal conditions suitable for the growth of tissue culture seedlings. The results showed that the explants of young culm from 1-year-old seedlings which were disinfected with 0.10%-0.15% mercuric chloride for 6-9 min and cultivated in standard MS medium with 1.5-2.0 g/mL of 6-BA, could achieve higher bud induction rate. When the 1-year-old tissue culture seedlings were transplanted, the seedlings were covered in greenhouse and cultivated with the fine particles made from plant debris, which could achieved higher survival rate and better growth.
钟永莉, 陈玟旭, 谭宏超. 云南甜龙竹组培及组培苗移栽技术研究[J]. 世界竹藤通讯, 2019, 17(2): 31-35.
Zhong Yongli, Chen Wenxu, Tan Hongchao. A Study of Tissue Culture and Seedling Transplanting Technology for Dendrocalamus brandiss. World Bamboo and Rattan, 2019, 17(2): 31-35.
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