Influencing Factors to Proliferation Culture of Bambusa multiplex Plantlets in vitro
Li Jia1, Ma Xiaoyue1, Zhang Xueying1, Gao Zhimin2
1 College of Horticulture, Agricultural University of Hebei, Baoding 071001, Hebei, China; 2 International Center for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102, China
Abstract：The in vitro plantlets of Bambusa multiplex were selected as test materials to study the effects of the basic media, plant growth regulators and the inoculation methods on the plantlet proliferation culture. The results indicated that the suitable medium formula for the plantlet proliferation culture was MS + 6-BA 2. 0 mg/L + 0. 4 mg/L NAA + activated carbon 1 g/L. The treatment of burying internodes into medium could increase the proliferation coefficient of the bamboo plantlets. Meanwhile, the removal of plantlet top could contribute to the enlargement of the nodes at lower parts, and thus induce the formation of adventitious roots, which could also lead to the improvement of proliferation coefficient.
李佳, 马晓月, 张学英, 高志民. 孝顺竹组培苗增殖培养影响因素探讨[J]. 世界竹藤通讯, 2016, 14(4): 5-9.
Li Jia, Ma Xiaoyue, Zhang Xueying, Gao Zhimin. Influencing Factors to Proliferation Culture of Bambusa multiplex Plantlets in vitro. World Bamboo and Rattan, 2016, 14(4): 5-9.