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世界竹藤通讯  2016, Vol. 14 Issue (4): 5-9     https://doi.org/10.13640/j.cnki.wbr.2016.04.002
  学术园地 本期目录 | 过刊浏览 | 高级检索 |
孝顺竹组培苗增殖培养影响因素探讨
李佳1, 马晓月1, 张学英1, 高志民2
1 河北农业大学园艺学院 河北保定 071001;
2 国际竹藤中心 国家林业局竹藤科学与技术重点开放实验室 北京 100102
Influencing Factors to Proliferation Culture of Bambusa multiplex Plantlets in vitro
Li Jia1, Ma Xiaoyue1, Zhang Xueying1, Gao Zhimin2
1 College of Horticulture, Agricultural University of Hebei, Baoding 071001, Hebei, China;
2 International Center for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102, China
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摘要 以孝顺竹组培苗为试材,研究了基本培养基、植物生长调节剂配比以及接种方式等对孝顺竹组培苗增殖培养的影响。结果表明,适宜孝顺竹增殖生长的培养基配方为MS+6-BA 2.0 mg/L+NAA 0.4 mg/L+活性炭1 g/L;对孝顺竹组培苗进行埋节处理可以提高增殖系数;同时孝顺竹组培苗去除顶梢后伤口下部竹节增大,有利于出现不定根,也可以提高增殖系数。
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李佳
马晓月
张学英
高志民
关键词 孝顺竹组培苗植物生长调节剂增殖    
Abstract:The in vitro plantlets of Bambusa multiplex were selected as test materials to study the effects of the basic media, plant growth regulators and the inoculation methods on the plantlet proliferation culture. The results indicated that the suitable medium formula for the plantlet proliferation culture was MS + 6-BA 2. 0 mg/L + 0. 4 mg/L NAA + activated carbon 1 g/L. The treatment of burying internodes into medium could increase the proliferation coefficient of the bamboo plantlets. Meanwhile, the removal of plantlet top could contribute to the enlargement of the nodes at lower parts, and thus induce the formation of adventitious roots, which could also lead to the improvement of proliferation coefficient.
Key wordsBambusa multiplex    plantlets in vitro    plant growth regulator    proliferation
     出版日期: 2018-05-21
基金资助:林业公益性行业科研专项(编号:201504106)。
通讯作者: 张学英,女,教授,硕士生导师,研究方向为果树生物技术。E-mail:zhangxueying1996@163.com;高志民,男,研究员,博士生导师,研究方向为竹藤生长发育的分子基础。E-mail:gaozhimin@icbr.ac.cn     E-mail: zhangxueying1996@163.com;gaozhimin@icbr.ac.cn
作者简介: 李佳,女,在读硕士生,从事果树生物技术研究。E-mail:lijia1203@163.com。
引用本文:   
李佳, 马晓月, 张学英, 高志民. 孝顺竹组培苗增殖培养影响因素探讨[J]. 世界竹藤通讯, 2016, 14(4): 5-9.
Li Jia, Ma Xiaoyue, Zhang Xueying, Gao Zhimin. Influencing Factors to Proliferation Culture of Bambusa multiplex Plantlets in vitro. World Bamboo and Rattan, 2016, 14(4): 5-9.
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http://www.cafwbr.net/CN/10.13640/j.cnki.wbr.2016.04.002      或      http://www.cafwbr.net/CN/Y2016/V14/I4/5
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