Synthesis and Expression Vector Construction of amiRNA Precursor for DlSCL6 in Dendrocalamus latiflorus
Chen Dongliang1,2, Sun Huayu2, Li Lichao2, Zhao Hansheng2, Gao Zhimin2
1 Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 2 International Center for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102, China
Abstract：Web MicroRNA Designer (WMD3) is a professional website specially used for sequence design of plant artificial miRNA and its precursor. Overlap PCR method was used for the synthesis of amiRNA precursor sequence, which involved multiple PCR purifications and recovery processes with complicated steps and a longer period. In this study, sequences of plant artificial miRNA and its precursor for DlSCL6 in Dendrocalamus latiflorus were designed according to WMD3 and an improved method of overlapping PCR was used for quickly cloning of the precursor sequence. Based on the frame of pCMABIA1301 vector, the expression vector of amiRNA precursor for DlSCL6 was constructed, which laid a foundation for further study in the expression regulation of SCL6 using amiRNA.
陈东亮, 孙化雨, 李利超, 赵韩生, 高志民. 麻竹DlSCL6基因amiRNA前体合成及表达载体构建[J]. 世界竹藤通讯, 2016, 14(2): 1-5.
Chen Dongliang, Sun Huayu, Li Lichao, Zhao Hansheng, Gao Zhimin. Synthesis and Expression Vector Construction of amiRNA Precursor for DlSCL6 in Dendrocalamus latiflorus. World Bamboo and Rattan, 2016, 14(2): 1-5.
