Abstract：The soil-covering mushroom spawn cultivation and casing methods are experimented with different matrix formulas and different selenium content under sympodial bamboo forest. The results show that cultivation matrix has a significant effect on the yield and nutrients of Dictyophora indusiate, and two casing methods of three-line shallow grooves and double turtle-back shape are conducive to improving the yield of D. indusiate. The yield of improved matrix increases by about 50% compared with conventional matrix. The yield increases first with the increase of selenium concentration and then decreases when the content of added selenium falls in the range of 0-2.0 mg/kg. By adding 1.5 mg/kg selenium in matrix, the yield is 195.30% higher than that without selenium adding. Adding 1.0-2.0 mg/kg selenium in the matrix could significantly increase averagely the selenium content in D. indusiate flowers, under which the selenium content in dry mass could grow from 2.50 mg/kg to 8.05-13.30 mg/kg, and the utilization rate of added selenium fertilizer could be 9.70%-15.36%. Different cultivation matrix formulas have a significant effect on content of crude polysaccharide and crude protein in D. indusiate fruiting bodies. The crude protein content in D. indusiata eggs and flowers in the improved matrix is 1.21 and 1.29 times higher than those in conventional cultivation matrix, and the crude polysaccharide is 4.81 and 1.35 times higher. The crude protein content of D. indusiata eggs and flowers with selenium adding in matrix is 40.90% and 14.30% higher than those without selenium adding. The per-unit-area yield of fresh D. indusiata eggs is the highest if the casing method of three-line shallow grooves is used with the selenium content at 1.0 mg/kg, amounting to 10.27 kg/m2, while fresh D. indusiata eggs on a single spawn are the most with the casing method of double turtle-back shape, reaching 1.40 kg. The biotransformation rates of the two casing methods are 93.00% and 94.14%, respectively, which reach a higher level.